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. Author manuscript; available in PMC: 2016 Nov 26.
Published in final edited form as: Nature. 2015 Nov 9;527(7579):477–483. doi: 10.1038/nature15699

Extended Data Figure 9. Screening of ZINC compounds predicted to be active at GPR65 based on BTB09089 docking poses.

Extended Data Figure 9

Primary screening with ZINC compounds (30 μM) for agonist activity at GPR65 when receptors were kept inactive at pH 8.40 (a); at control HEK293 T cells for nonspecific activity (b); at GPR65 when receptors were activated at pH 7.40 for modulator or antagonist activity (c); at GPR65 when receptors were activated by BTB09089 (30 μM) at pH 8.40 for modulator or antagonist activity (d). Normalized results represent mean ± SM from a minimum of three assays (each in minimum of triplicate and a total of ≥ 16 measurements). The red dashed line in panel b – d indicates the 20% inhibition line - an arbitrary cut-off line.