Skip to main content
. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: Anticancer Res. 2011 Dec;31(12):4099–4109.

Figure 2.

Figure 2

Comparison of memory phenotype marker expression on CD8+ T-cells between IL-2-TIL microcultures and anti-CD3+IL-2-TIL samples from melanoma patients. IL-2-TIL microcultures represent TIL samples which were initiated from the tumor specimens resected from melanoma patients in the presence of high-dose interleukin-2 (IL-2) for two or three weeks. Anti-CD3+IL-2-TIL samples refer to the TIL samples for adoptive cell transfer after using the rapid expansion protocol in the presence of high-dose IL-2, anti-CD3 antibody and allogeneic peripheral blood mononuclear cells for two weeks. T-Cells from both IL-2-TIL microculture samples and anti-CD3-IL-2-TIL samples were co-stained with R-phycoerythrin-conjugated anti-human CD27, CD28, CD62L and fluorescein isothiocyanate-conjugated anti-human CD8 for FACS analysis. The percentage of CD8+CD62L+ (A), CD8+CD27+ (B) and CD8+CD28+ (C) T-cells from both IL-2-TIL microculture samples and anti-CD3+IL-2-TIL samples were calculated and compared.