Coexpression of CD44 and the memory phenotype markers on T-cells in a representative anti-CD3+IL-2-TIL sample from a melanoma patient. T-Cells from anti-CD3+IL-2-TIL sample were stained with R-phycoerythrin (PE)-conjugated anti-human CCR7, CD62L, CD27, CD28 monoclonal antibodies and fluorescein isothiocyanate (FITC)-conjugated anti-human CD44 monoclonal antibody and then flow cytometric analysis was performed to determine the association between CD44 expression and the memory phenotype marker expression on T-cells. Coexpression of CD44 and the memory phenotype markers on T-cells from a representative TIL sample derived from a melanoma patient is shown. The x-axis represents the fluorescence staining of CD44, and the y-axis represents CCR7 (A), CD62L (B), CD27 (C) and CD28 (D) staining, respectively.