Figure 3. Zinc sparks are highly correlated with the total cell number of late stage pre-implantation embryos.

(A) (a) Cell numbers of the embryos that were not imaged during fertilization (non-imaged); (b) amplitude of zinc sparks (empty bars) and total cell numbers (solid bars) in the embryos that arrested at morula stage (blue) or reached blastocyst stage (red) 120 hours post zinc imaging. (B) Z scores, which is calculated as (x-xbar)/SD, of total cell number of embryos and the z scores of the zinc spark amplitude in the imaged embryos from (A) are highly correlated. (*p < 0.05, ***p < 0.001).