Figure 5.

Pretreatment of HAEC with antibodies against α5 and β1, but not α3, αv, or β3, inhibited MM-LDL–mediated monocyte binding. HAEC were pretreated for 30 min with 5 μg/ml of blocking antibodies to α5, α3, αv, β1, and β3 before addition of MM-LDL for 4 h at 37°C (in the continuing presence of antibody). The cells were rinsed extensively, and a monocyte binding assay was performed. The MM-LDL–induced increase in monocyte binding was significantly blocked back to control levels by antibodies to α5 and β1 but not α3, αv, or β3. The data presented are representative of four experiments. Values represent mean ± SD (n = 12). *P < 0.001.