Figure 7.
Confocal microscopy of HAEC stained for HUTS-21. HAEC were either untreated (a), or treated with MM-LDL for 4 h (b) or with 1.5 mM MnCl2 for 15 min (c). Binding of the HUTS-21 antibody was viewed using CY3-conjugated secondary antibody (red). Confocal microscopy determined the β1 staining to be restricted to the apical surface. The control cells displayed a few clusters of activated β1, whereas cells stimulated with MM-LDL demonstrated an increase in these clusters. MnCl2 served as positive control, and a diffuse increase in staining with fewer and smaller clusters can be seen. ×500.