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. 2016 Mar 18;9:160. doi: 10.1186/s13071-016-1442-4

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© Ratnappan et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RNAi mediated phenotype and transcript changes in H. bacteriophora injected with nol-5 dsRNA. Adult H. bacteriophora hermaphrodites that were injected with nol-5 dsRNA produced progeny with no germline and empty gonad. a. Progeny of non-injected H. bacteriophora. The area with white dotted lines indicates the position of the gonads. b. Progeny of H. bacteriophora injected with nol-5dsRNA. c. Expression of nol-5 gene in the progeny of nol-5 dsRNA injected worms. The y-axis represents the fold change in mRNA expression in the progeny of H. bacteriophora hermaphrodites. The mRNA levels are normalized to nol-5 expression in the progeny of non-injected hermaphrodites (black bar). The green bar represents nol-5 levels in progeny of gfp injected worms. The red bar represents nol-5 levels in phenotypically wild type siblings, and the blue bar represents the nol-5 levels in worms with empty gonads. To control for off-target effects, expression of an unrelated gene (cct-2) in worms with empty gonads was determined (brown bar). The graph was obtained by combining data from at least three independent biological replicates. Error bars indicate the standard error of the mean. Asterisks depict the statistical significance of the observed differences in unpaired, two-tailed t-tests with P-values < 0.001 (**)