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. 2016 Mar 18;13:24. doi: 10.1186/s12986-016-0081-2

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© Garcia et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Thermoregulatory genes and white adipocyte markers in vitro. The transcripts for UCP1 a, CIDEA b, Cox8b c, Serpina3k d, Wdnm1-like e, and ASC1 (f) were evaluated in white and beige adipocytes in culture treated with isoproterenol (+ Iso) or without (− Iso) for 6 hrs. before harvesting the cells. Thermoregulatory gene transcripts were normalized to white –Iso, whereas white adipocyte gene transcripts were normalized to beige – Iso. Values are means ± SEM (n = 3). (*) different from white – Iso, (#) different from white + Iso, (+) different from beige + Iso, (@) different from beige – Iso, p ≤ 0.05, two way ANOVA followed by multiple comparisons using the Holm-Sidak method