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. 2016 Mar 17;17:247. doi: 10.1186/s12864-016-2575-8

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© Bull et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of the HCV quasispecies utilising near full-length reads with the PacBio RS platform. Individual sequences reads greater than 9 kb were generated and aligned for an HCV infected individual, RIMM. Panel (a). Highlighter plot (www.hiv.lanl.gov/content/sequence/HIGHLIGHT/highlighter.html) representing 45 unique haplotypes that were detected from sequence reads. The name of each haplotype shows the frequency of occurrence within the population (e.g. HAP_0.11). The master sequence on the top of the plot is the consensus genome sequence obtained from the Illumina sequences. Panel (b). Phylogenetic tree showing the genetic relationship between near-full length quasispecies obtained from PacBio reads