FIG. 3.
Expression of a TCR transgene abolishes Vβ13 SJ formation in promoter-mutant mice. (A) Analysis of Vβ13 SJ in various types of mice. Vβ13-to-Dβ1 or -to-Dβ2 SJ were assayed by PCR in thymocyte DNA from RAG2−/− mice or +/+, P13−/−, and P13R/R mice in the presence or absence of the 2C TCR transgene. Half of the PCR products were digested with ApaLI before separation on agarose gels. PCR products were hybridized with a Vβ13-specific oligonucleotide probe. Shown are representative data from three experiments. (B) Comparison of the levels of Vβ13 SJ in wild-type and promoter-mutant mice. DNA samples were serially diluted and then used to assay for Vβ13-to-Dβ1 or -to-Dβ2 SJ. Numbers indicate the band intensities normalized to that of JAK3. The level of Vβ13 SJ in wild-type DNA was arbitrarily defined as 1. Shown are representative data from three experiments.