TABLE 2.

PCR conditions for assaying Vβ GT

TCR Vβ segment	RT-PCR conditions	Nested PCR conditions
Vβ8.1	Primers Vβ8.1F and Vβ8.1R1; 60°C; 15×	Primers Vβ8.1F and Vβ8.1R2; 62°C; 26×
Vβ12	Primers Vβ12F1 and Vβ12R; 56°C; 15×	Primers Vβ12F2 and Vβ12R; 58°C; 27×
Vβ13	Primers Vβ13F and Vβ13R1; 60°C; 15×	Primers Vβ13F and Vβ13R2; 62°C; 28×
β-actin	Primers B-F and B-R; 60°C; 24×