Table 1.
Primers and amplification conditions used for PCR
Primer | Oligonucleotide sequence (5′–3′) | Gene | Amplification conditions | Product size (bp) | References |
---|---|---|---|---|---|
P90, P91 | GTTCGGGGCCGTCGCTTAGG, GAGGTCGATCGCCCACGTGA | IS900 | 95 °C for 5 min, then 34 cycles of 95 °C for 1 min, 58 °C for 1.5 min, 72 °C for 1.5 min. Final extension of 10 min at 72 °C | 398 | Naser et al. [3] |
AV1, AV2 | ATGTGGTTGCTGTGTTGGATGG, CCGCCGCAATCAACTCCAG | IS900 | 95 °C for 5 min, then 34 cycles of 95 °C for 1 min, 58 °C for 1.5 min, 72 °C for 1.5 min. Final extension of 10 min at 72 °C | 298 | Naser et al. [3] |