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. 2004 Aug;24(16):7249–7259. doi: 10.1128/MCB.24.16.7249-7259.2004

FIG. 3.

FIG. 3.

Sgf11p copurifies with the SAGA subunits Gcn5p and Spt7p. Immunoaffinity copurification assays were performed with yeast strains containing either a plasmid expressing V5-tagged-SGF11 (pYes-SGF11) or the empty vector (pYes-DEST52). Proteins were analyzed by immunoblotting (IB) as follows. The numbers on the left of each panel represent molecular weight standards. (A) Cell lysates were probed with an anti-V5 antibody. The V5 fusion protein is seen at the expected molecular mass of 13 kDa. (B and C) Proteins immunoaffinity purified (IP) with anti-Gcn5p and anti-Spt7p antibodies, respectively, were probed with an anti-V5 antibody. V5-Sgf11p was recovered with both Gcn5p and Spt7p. (D) Proteins purified with protein A-Sepharose alone were probed with an anti-V5 antibody. No V5-Sgf11p was recovered. (E) Proteins immunoaffinity purified (IP) with an anti-V5 antibody were probed with an anti-Spt7p antibody. Spt7p was recovered with V5-Sgf11p.