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Sgf11p is not required for SAGA's HAT activity. SAGA complexes were immunoaffinity purified from the indicated strains with an anti-Gcn5p antibody and used in HAT assays containing nucleosomal histones and 3H-acetyl-CoA. (A) Aliquots from each HAT assay were subjected to SDS-PAGE, followed by autoradiography to reveal labeled histones. (B) Aliquots of the same reactions were subjected to immunoblotting (IB) with the anti-Gcn5p antibody to show recovery of Gcn5p.
