TABLE 2.

Mass spectrometry analysis of SAGA purified from wild-type and sgf11 deletion strains^a

ORF	Protein	WT avg NPAF	sgf11Δ avg NPAF	NPAF sgf11Δ/WT ratio
YGR252W	TRA1	0.00624	0.00475	0.7613
YDR145W	SPT7	0.00661	0.00621	0.94
YLR055C	TAF5	0.00649	0.00749	1.1529
YOL148C	ADA3	0.01354	0.01425	1.0523
YDR448W	SGF73	0.00774	0.00961	1.2419
YGL066W	SPT20	0.00599	0.00817	1.363
YCL010C	SPT8	0.00634	0.00905	1.4278
YBR198C	TAF12	0.0054	0.00935	1.7327
YGL112C	TAF6	0.00607	0.00626	1.0307
YDR176W	ADA1	0.00711	0.00467	0.6565
YDR392W	UBP8	0.00686	0	0
YBR081C	GCN5	0.00405	0.00875	2.1584
YMR236W	ADA2	0.01256	0.01597	1.2718
YHR099W	SPT3	0.00738	0.007	0.9477
YPL254W	SGF29	0.01307	0.01538	1.1766
YDR167W	TAF10	0.00545	0.00343	0.6288
YMR223W	TAF9	0.00708	0.00587	0.8289
YPL047W	SGF11	0.00458	0	0

^aProteins immunoaffinity purified with Gcn5p from the wild type (WT) and from a sgf11Δ deletion strain were identified by DALPC mass spectrometry. To estimate the relative composition of individual components in purified SAGA, the PAF value for each subunit was normalized to the total number of nonredundant spectra identifying the entire SAGA complex (see Materials and Methods). We call this value the normalized PAF (NPAF). The table shows the average NPAF values from duplicate experiments and the ratio of these averages from the two strains. The overall average ratio ± its SD was 1.02 ± 0.527.