Table 3.
Substrate | Acinetobacter sp. ASC1 | Pseudomonas sp. ASC2 | Enterobacter sp. ASC3 | Bacillus sp. ASC4 | ||||
---|---|---|---|---|---|---|---|---|
CDM (g/L) | PHAs (wt %) | CDM (g/L) | PHAs (wt %) | CDM (g/L) | PHAs (wt %) | CDM (g/L) | PHAs (wt %) | |
Glucose | 2.7 ± 0.1 | 8.2 ± 3.5 | 2.2 ± 0.1 | 21.7 ± 2.6 | 2.6 ± 0.2 | 16.8 ± 1.9 | 4.8 ± 0.4 | 18.9 ± 1.5 |
Refined glycerol | 8.6 ± 0.1 | 3.7 ± 2.4 | 10.0 ± 0.2 | 3.9 ± 0.9 | 7.2 ± 0.1 | 14.1 ± 2.7 | 6.1 ± 0.2 | 6.7 ± 2.8 |
Crude glycerol | 8.5 ± 0.2 | 25.4 ± 3.1 | 10.7 ± 0.1 | 28.2 ± 1.2 | 9.8 ± 0.3 | 33.7 ± 1.3 | 7.8 ± 0.1 | 34.4 ± 3.4 |
Canola oil | 9.0 ± 0.1 | 2.2 ± 3.2 | 12.1 ± 0.2 | 2.4 ± 2.0 | 9.2 ± 0.1 | 1.8 ± 1.4 | 9.5 ± 0.2 | 2.5 ± 1.1 |
Palm oil | 13.3 ± 0.4 | 14.2 ± 3.5 | 17.8 ± 0.3 | 5.5 ± 1.2 | 12.1 ± 0.1 | 12.0 ± 2.2 | 14.6 ± 0.3 | 12.1 ± 2.0 |
Soybean oil | 5.8 ± 0.3 | 6.7 ± 2.0 | 9.7 ± 0.1 | 5.6 ± 2.2 | 8.1 ± 0.2 | 5.3 ± 1.8 | 6.6 ± 0.1 | 3.4 ± 1.3 |
Sunflower oil | 8.4 ± 0.1 | 7.3 ± 2.1 | 7.2 ± 0.1 | 2.5 ± 1.8 | 6.5 ± 0.3 | 5.5 ± 2.0 | 7.9 ± 0.1 | 4.7 ± 0.9 |
Corn oil | 5.9 ± 0.2 | 4.1 ± 1.7 | 5.1 ± 0.1 | 2.6 ± 1.5 | 5.2 ± 0.1 | 3.1 ± 1.4 | 5.8 ± 0.3 | 2.2 ± 1.0 |
Grape seed oil | 5.3 ± 0.1 | 7.4 ± 1.9 | 10.2 ± 0.2 | 5.6 ± 2.5 | 6.1 ± 0.1 | 6.3 ± 1.0 | 6.2 ± 0.1 | 5.1 ± 1.3 |
Olive oil | 5.3 ± 0.1 | 5.0 ± 1.0 | 5.3 ± 0.1 | 3.7 ± 0.8 | 4.3 ± 0.2 | 2.1 ± 0.8 | 5.0 ± 0.1 | 3.2 ± 0.7 |
Rice bran oil | 9.9 ± 0.1 | 5.1 ± 1.2 | 17.0 ± 0.2 | 5.9 ± 1.9 | 10.1 ± 0.1 | 4.8 ± 1.1 | 12.5 ± 0.1 | 6.0 ± 1.5 |
Camellia seed oil | 5.7 ± 0.1 | 3.4 ± 1.1 | 5.5 ± 0.1 | 3.3 ± 1.1 | 4.8 ± 0.1 | 2.9 ± 0.6 | 3.7 ± 0.1 | 2.2 ± 0.2 |
Bacteria were cultured in production medium with 20 g/L carbon source at a carbon to nitrogen ratio of 200 for 72 h at 30 °C on a rotary shaker. The dry cell mass was assayed by GC analysis. All data are expressed as ± SD and represent the mean value of three parallel experiments
CDM cell dry mass (g/L), PHA total PHA content (wt %)