Table 1. Gapmer ASO sequences and their on-target mRNA transcripts.

Isis No	Alternative ID	Sequence	Modification	Gene	Genbank number	On-target Species	First Toxic Dose (mg/kg) >1000 IU/ml ALT (96 h)
569713	ASO ctrl	5′-GACGCGCCTGAAGGTT-3′	LNA	N/A, control	N/A	Mouse	>300
571035	FVII-1	5′-CAGATATAGGACTGGA-3′	LNA	F7	NM_010172.3	Human	>300
571033	FXI-3	5′-ATCCAGAGATGCCTCC-3′	LNA	F11	NM_028066.1	Mouse	>300
569714	FXI-4	5′-GGCCACCACGCTGTCA-3′	LNA	F11	NM_028066.1	Mouse	>300
571034	FXI-5	5′-TGCCACCGTAGACACG-3′	LNA	F11	NM_028066.1	Mouse	>300
569715	SOD1–1	5′-GGACACATTGGCCACA-3′	LNA	Sod1	NM_011434.1	Mouse	>300
569716	FVII-2	5′-CCCTGGTGTACACCCC-3′	LNA	F7	NM_010172.3	Mouse	300
569717	PTEN	5′-ATCATGGCTGCAGCTT-3′	LNA	Pten	NM_008960.2	Mouse	33
569718	FVII-3	5′-TGGTCCCTGCAGTACT-3′	LNA	F7	NM_010172.3	Mouse	100
569719	FXI-1	5′-GTCTGTGCATCTCTCC-3′	LNA	F11	NM_028066.1	Mouse	11
443919	FXI-1-M	5′-GTCTGTGCATCTCTCC-3′	2’ MOE	F11	NM_028066.1	Mouse	300
464917	FXI-1-C	5′-GTCTGTGCATCTCTCC-3′	cEt	F11	NM_028066.1	Mouse	11
569720	FXI-2	5′-GTCAGTATCCCAGTGT-3′	LNA	F11	NM_028066.1	Mouse	100
444011	FXI-2-M	5′-GTCAGTATCCCAGTGT-3′	2’ MOE	F11	NM_028066.1	Mouse	300
465178	FXI-2-C	5′-GTCAGTATCCCAGTGT-3′	cEt	F11	NM_028066.1	Mouse	100
569721	SOD1–2	5′-TGAGGTCCTGCACTGG-3′	LNA	Sod1	NM_011434.1	Mouse	33
529933	SOD1–2-M	5′-TGAGGTCCTGCACTGG-3′	2’ MOE	Sod1	NM_011434.1	Mouse	300
508031	SOD1–2-C	5′-TGAGGTCCTGCACTGG-3′	cEt	Sod1	NM_011434.1	Mouse	33
554219	BIRC5	5′- CTCAATCCATGGCAGC-3′	LNA	Birc5	NM_001168.2	Human	300

The 16-mer antisense oligonucleotides were synthesized with the indicated modifications (underlined sequences). The center (gap) of the antisense oligonucleotides (not underlined) consist of deoxynucleotide bases and phosphorothioate backbone linkages.