Skip to main content

FIG. 4.

FIG. 4.

Scrambled Ure2p prion domains form filaments. Prion domains were purified under denaturing conditions and dialyzed into water overnight at 4°C. Significant precipitate was visible for all constructs except Ure2-25p1-90. Precipitate was observed for Ure2-25p1-90 after further 12 h rotation at 4°C. Precipitates were stained with uranyl acetate and visualized by negative-staining electron microscopy. Bar, 100 nm.