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TABLE 1.

Formation, stability, and dominance of USA+ colonies in strains with integrated URE2 variants

Integrated URE2 Plasmida Mean no. of USA+ colonies/106 cellsb ± SEM USA+ stabilityc USA+ dominanced
URE2-20 Vector, pH317 1.3 ± 0.2 19/44 14/19
F.L., pER62 30 ± 6 12/22 11/12
P.D., pER63 225 ± 65 8/22 5/8
URE2-21 Vector, pH317 67 ± 27 2/44 2/2
F.L., pER95 220 ± 110 16/22 14/16
P.D., pER98 1970 ± 640 20/22 18/20
URE2-22 Vector 8.2 ± 1.5 0/44 0/0
F.L., pER112 21 ± 3 2/44 0/2
P.D., pER99 360 ± 68 0/44 0/0
URE2-23 Vector 1.3 ± 0.8 1/44 0/1
F.L., pER96 4.0 ± 2.0 1/22 1/1
P.D., pER100 7.7 ± 0.3 2/22 2/2
URE2-24 Vector 2.7 ± 1.0 16/36 12/16
F.L., pER113 60 ± 26 17/44 17/17
P.D., pER101 68 ± 27 37/44 34/37
URE2-25 Vector 2.7 ± 0.9 12/26 8/12
F.L., pER97 11 ± 2 2/22 2/2
P.D., pER102 70s ± 40 2/22 2/2
a

All strains carry a LEU2 plasmid containing the GAL1 promoter (vector, pH317) or a version of pH317 modified to express either a full-length version of the Ure2p variant (F.L.) or the prion domain of the Ure2p variant (P.D.).

b

That is, the number of USA+ cells per 106 cells after growth in galactose-raffinose dropout medium. Standard errors are indicated.

c

That is, the fraction of USA+ colonies that remained USA+ after 48 h of growth on YPAD.

d

That is, the fraction of stable USA+ colonies whose USA+ phenotype was dominant when mated with cells carrying the same URE2 variant and unable to utilize USA.