TABLE 1.
Formation, stability, and dominance of USA+ colonies in strains with integrated URE2 variants
Integrated URE2 | Plasmida | Mean no. of USA+ colonies/106 cellsb ± SEM | USA+ stabilityc | USA+ dominanced |
---|---|---|---|---|
URE2-20 | Vector, pH317 | 1.3 ± 0.2 | 19/44 | 14/19 |
F.L., pER62 | 30 ± 6 | 12/22 | 11/12 | |
P.D., pER63 | 225 ± 65 | 8/22 | 5/8 | |
URE2-21 | Vector, pH317 | 67 ± 27 | 2/44 | 2/2 |
F.L., pER95 | 220 ± 110 | 16/22 | 14/16 | |
P.D., pER98 | 1970 ± 640 | 20/22 | 18/20 | |
URE2-22 | Vector | 8.2 ± 1.5 | 0/44 | 0/0 |
F.L., pER112 | 21 ± 3 | 2/44 | 0/2 | |
P.D., pER99 | 360 ± 68 | 0/44 | 0/0 | |
URE2-23 | Vector | 1.3 ± 0.8 | 1/44 | 0/1 |
F.L., pER96 | 4.0 ± 2.0 | 1/22 | 1/1 | |
P.D., pER100 | 7.7 ± 0.3 | 2/22 | 2/2 | |
URE2-24 | Vector | 2.7 ± 1.0 | 16/36 | 12/16 |
F.L., pER113 | 60 ± 26 | 17/44 | 17/17 | |
P.D., pER101 | 68 ± 27 | 37/44 | 34/37 | |
URE2-25 | Vector | 2.7 ± 0.9 | 12/26 | 8/12 |
F.L., pER97 | 11 ± 2 | 2/22 | 2/2 | |
P.D., pER102 | 70s ± 40 | 2/22 | 2/2 |
All strains carry a LEU2 plasmid containing the GAL1 promoter (vector, pH317) or a version of pH317 modified to express either a full-length version of the Ure2p variant (F.L.) or the prion domain of the Ure2p variant (P.D.).
That is, the number of USA+ cells per 106 cells after growth in galactose-raffinose dropout medium. Standard errors are indicated.
That is, the fraction of USA+ colonies that remained USA+ after 48 h of growth on YPAD.
That is, the fraction of stable USA+ colonies whose USA+ phenotype was dominant when mated with cells carrying the same URE2 variant and unable to utilize USA.