FIG. 6.

Survival of wild-type and PARG^Δ2-3/Δ2-3 mice in response to DNA damage. (A) Seven-week-old mice with the indicated genotypes received a single dose of MNU (150 mg/kg of body weight) at day 0. For treatment with MNU, six males and six females of the PARG^Δ2-3/Δ2-3 and wild-type backgrounds and two males and four females of the PARP-1^−/− background were used. The percentage of viable mice is plotted against age. The mean survival of MNU-treated PARG^Δ2-3/Δ2-3 mice was 14.75 weeks of age. compared to 19.42 weeks for wild-type mice (P = 0.017). The onset of death of PARG^Δ2-3/Δ2-3 mice after MNU treatment is significantly earlier compared to wild-type controls (P < 0.05). (B) Mice at 7 to 9 weeks of age were exposed to 10 Gy of whole-body γ irradiation. For wild-type mice, seven males and six females were used; for PARG^Δ2-3/Δ2-3 mice, eight males and eight females were used. The percentage of viable mice is plotted against age. n, number of mice in each group. The mean survival of PARG^Δ2-3/Δ2-3 mice after irradiation was 13.19 weeks, compared to 15.77 weeks for wild-type mice (P = 0.046).