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. 2015 Dec 31;44(5):2214–2226. doi: 10.1093/nar/gkv1526

Figure 3.

Figure 3.

HDGFRP2 is essential for the recruitment of RBBP8/CtIP DNA DSBs. (A) Representative confocal images of U2OS GFP-RBBP8/CtIP cells transfected with indicated siRNAs for 72 h and subjected to micro-laser irradiation followed by 15 min incubation (left), and quantification of the percentage of GFP-RBBP8/CtIP-stripe forming cells of all micro-irradiated cells (right). Error bars, SEM of three independent experiments. *** P < 0.005 when compared with cells transfected with control siRNA in parallel. Scale bar, 10 μm. (B) Representative confocal images of U2OS GFP-RBBP8/CtIP cells transfected with indicated siRNAs for 72 h, irradiated as in (A) and stained with indicated antibodies and DAPI (nuclei) (left), quantification of the percentage of GFP-RBBP8/CtIP-stripe forming Cyclin A2-positive cells of all Cyclin A2-positive micro-irradiated cells (right, upper panel) and immunoblots of indicated proteins from the cell lysates (right, lower panel). Scale bar, 20 μm.