Figure 1.

Functional complementation of the mgm101–1^ts mutation in Saccharomyces cerevisiae with CpMgm101. S. cerevisiae M2915–7C cells transformed with the indicated plasmids were cultivated for 2 days in SGal medium at permissive (25°C) or restrictive (37°C) temperatures. The cultures were spotted in serial dilutions onto SGly plates and cultivated at the indicated temperatures for 7 days. pYES2 and pYES2/CT are empty vectors while pYES2-ScMgm101 and pYES2-CpMgm101 contain ScMgm101 and CpMgm101, respectively.