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. 2016 Jan 6;44(5):2227–2239. doi: 10.1093/nar/gkv1529

Figure 3.

Figure 3.

CpMgm101 binds to a range of DNA structures with free 3′ or 5′ ends. The EMSA experiments were performed with either CpMgm101 or CpMgm101-Δ84 (concentration is expressed per monomer) and 3 nM of the corresponding DNA substrate at 30°C for 10 min (see ‘Materials and Methods’ section). Lane 1 shows a control containing the DNA probe without CpMgm101. Samples were separated in 0.6% agarose gel in 0.5× TBE. (A and B) The DNA binding properties of CpMgm101 to different DNA substrates. (1) control, (2) 0.5 μM CpMgm101, (3) 1 μM, (4) 2 μM, (5) 3 μM, (6) 5 μM, (7) 2 μM + 1 μl anti CpMgm101 IgG antibodies (B only). The addition of anti-CpMgm101 antibodies to the CpMgm101–DNA reaction mixture resulted in a supershift, this complex is too large to enter the 0.6% agarose gel and the entire DNA probe remains in the well (lane 7). (C) The DNA binding properties of CpMgm101-Δ84 to different DNA substrates. (1) control, (2) 0.5 μM, (3) 1 μM, (4) 2 μM and (5) 5 μM.