Figure 2.

Schematic diagram illustrating the cloning strategy of promoter library (A) Cloning of combinatorial promoter library in pNYL-GFP/IbsC vector harboring GFP and toxic peptide IbsC as reporter genes. (B) A panel of synthetic constitutive promoter showing a wide range of promoter activity. (C)Toxic peptide IbsC based screening led to discovery of a clone which showed inducible nature with 5 μg/ml of nalidixic acid (sub-growth inhibitory concentration of nalidixic acid toward E. coli RFM 443), and growth profile (kinetic) analysis of the cells containing the isolated promoter in the presence of nalidixic acid.