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. 2016 Feb 2;44(5):2451–2461. doi: 10.1093/nar/gkw029

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Growth pattern of various stress related gene regulator knock out strains of E. coli harboring pNal::ibsC-amp and pRx::ibsC-amp fusion system in liquid and solid growth medium. (A) The growth assay of E. coli mutant strains in liquid LB media. A₆₀₀ measurements were normalized against the average A₆₀₀ corresponding to positive and negative controls. (B) The growth assay of E. coli mutant strains on LB agar. Null mutant of hns harboring pNal::ibsC plasmid showed scanty colonies and a retarded growth in broth and plate due to expression of toxic peptide IbsC in E. coli cells whereas, the control pRx::ibsC cells did not show significant growth difference.