Figure 5.

Biding of TFs inside operons. After SELEX screening of DNA-binding sequences for a total of116 TFs, some operons were found to carry the binding sites for specific TFs. Some representative operons are shown: (A) hyfA operon (13 581 bp); (B) sdhC operon (9872 bp); (C) wcaC operon (13 390 bp); and (D) nuoA operon (15 024 bp). Some TFs with clear binding peaks are shown, but there were several small peaks within all four operons. The binding level of the general silencer H-NS is also shown (blue line) even though the peak height is low. The fluorescent intensity of SELEX fragment binding to each probe relative to that of library DNA was determined, and is shown as the relative value to that of the highest peak.