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. 2016 Feb 11;44(5):2274–2282. doi: 10.1093/nar/gkw076

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Genomic mutations in target sites of PR-1611 inhibit RNAa. (A) Sequence of promoter regions containing PR-1611 target sites from wild-type HEK293A (WT) and mutated cells (Clone-5). Genome editing was confirmed by sequencing the PCR product from genomic DNA. The WT PR-1611 target site is shown in bold. The deletions are shown as dashes and the insertion is shown underlined.(B) Plot of PR mRNA expression in HEK293A cells after transfection with PR-11, PR-891, PR-1610 or PR-1611. (C) Comparison of PR activation in Clone-5 and WT cells induced by PR-11, PR-891, PR-1610 or PR-1611. PR mRNA expression levels were assessed by qPCR and normalized to GAPDH. The results are means ± SEM of at least three independent experiments and plotted as relative expression compared to NC transfection. **P < 0.001.