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. 2016 Mar 18;7:330. doi: 10.3389/fmicb.2016.00330

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Copyright © 2016 Jamil, Habib, Abbasi, Ihsan, Nasir and Imran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colony forming unit (CFU) assay. CFU assay was performed to count the viable bacteria after reaction of CSNPs and drug loaded CSNPs with pathogens. CFU was done by plating 10μL of sample from each test tube after serial dilutions in normal saline (0.9% NaCl). All plates were then incubated for 48 h and colonies were counted manually. CSNP, Chitosan nano-particles, NAB, Nano-antibiotic; KP, Klebsiella pneumoniae; Pseudo, Pseudomonas aeruginosa and MRSA for Methicillin-resistant S. aureus.