FIG. 2.

Inhibition of CPD repair by NER and photolyase (PR) in CEN14. (A) NER and PR at room temperature (RT). UCC510 cells were irradiated with 150 J of UV light/m²and exposed to photoreactivating light (lanes 3 to 12) or incubated in the dark (lanes 13 to 20). Lanes 1 and 2 contain DNA of nonirradiated cells. The DNA was cut with T4-Endo V (+) or was mock treated (−) and processed as described in the legend to Fig. 1. (B) UV irradiation and incubation in the dark were performed at 30°C. (C) Quantitative analysis of NER and PR at room temperature (RT) and NER at 30°C. The curves for experiments done at RT represent averages (± standard deviations) of results for four gels. For details about the schematic diagrams shown in panels A and B, see the legend to Fig. 1.