FIG. 3.

BTB and IVR domains contribute to degradation of Nrf2 by Keap1. (A) Schematic presentation of Keap1 deletion mutants. Keap1 contains mainly three characteristic domains, the BTB, IVR, and DGR domains. (B) Deletion of the BTB and IVR domains abolished Nrf2 degradation in the in vivo degradation assay (top). The experimental procedure is described in the legend to Fig. 1. The expression of cotransfected EGFP was used as an internal control (bottom). (C) The expression of Keap1 deletion mutants was also monitored by immunoblot analysis with two anti-Keap1 antibodies against the C-terminal and N-terminal ends (lanes 1 to 4 and lanes 5 to 7, respectively). wt, wild type.