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The BTB domain of Bach1 does not bind Cul3. Whole-cell extracts prepared from 293T cells transfected with expression plasmids (3 μg) in the combinations indicated were subjected to immunoprecipitation (IP) with anti-Flag antibody-conjugated beads followed by immunoblot (IB) analysis with an anti-Myc antibody (top). The expression level of each protein was monitored by immunoblot analysis with anti-Myc (middle) and anti-Flag (bottom) antibodies, respectively.
