Fig. 6.

SPIKE markers. a-c Agarose gel images analyzed by three SPIKE markers from the parental lines. The SPIKE-01SNP marker discriminated G/A SNP located on the third exon using two separated PCRs (SPIKE-01SNP- GF/R primer set and SPIKE-01SNP-AF/R primer set) (a). The SPIKE-03SNP marker detected the G/A SNP on the fifth exon using two separated PCRs (SPIKE-03SNP-F/GR primer set and SPIKE-03SNP-F/AR primer set) (b). The SPIKE-indel3 marker distinguished a 20-bp indel located in the promoter region (c). YP9 was used as a donor line of SPIKE gene. d-f Application of the SPIKE-01SNP marker (d), the SPIKE-03SNP marker (e), and the SPIKE-indel3 marker (f) in the intermediate breeding line. Thirteen BC₂F₁ plants derived from PSB Rc82 x YP9 cross were genotyped by the three markers, respectively