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. 2016 Mar 18;130(9):667–681. doi: 10.1042/CS20150622

Figure 5. AMPK-dependent p38 activation is involved in IL-1β-induced FGF-2 expression.

Figure 5

ATDC5 cells were incubated with IL-1β for the indicated times, and p38 phosphorylation was examined by Western blotting (A; n=7). ATDC5 cells were pre-treated with SB203580 (SB.) or cells were transfected with p38 siRNA for 12 h, and then stimulated with IL-1β for 24 h. FGF-2 expression was examined by Western blotting (B and C; n≥7) and ELISA (D; n=6). ATDC5 cells were pre-treated with DPI, NAC, Ara A or Compound C for 30 min or cells transfected with AMPK (α1 and α2) and p38 siRNA for 16 h, and then stimulated with IL-1β for 30 min. Phosphorylation of p38 (E and F; n≥8) and AMPK (G; n=6) was examined by Western blotting. Quantification results are expressed as means±S.D. *P<0.05 compared with the Con group (control); #P<0.05 compared with the IL-1β-treated group; $P<0.05 compared with the control siRNA-transfected group (D). Molecular masses are indicated in kDa. β-Actin was used as a loading control.