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Farnesyltransferase inhibition permits an intermediate level of ERK activation in MSCV-H-Ras-Neo-transduced human Lin− CB cells. After 10 days of culture under selection, the levels of H-Ras, ERK1/2, and phosphorylated ERK1/2 in transduced cells were determined by Western blotting of cell lysates. The ratios of phosphorylated ERK1/2 to total ERK1/2, normalized to levels in the control, are indicated.
