Table 2.
WNV-RNA concentration, copies/mla | Reactive samples/number tested | Percent positive |
---|---|---|
179 | 24/24 | 100 |
89.5b | 24/24 | 100 |
44.8 | 24/24 | 100 |
22.4 | 19/24 | 75 |
11.2 | 12/24 | 50 |
5.6 | 5/24 | 21 |
WNV-RNA concentration, copies/ml | ||
95 % detection limit (95% CI) | 30.2 (24.2-45.4) | |
Intra-assay WNVb, mean ±SD / CV | 33.20 ± 0.42 / 1.27 | |
Interassay WNVb, mean ±SD / CV | 32.62 ± 0.86 / 2.63 |
NATtrol(tm) WNV (NATWNV-0005, lot number 309897); calibrated to Health Canada WNV Reference preparation.
Intra- and interassay variabilities were calculated exemplarily for the concentration of 89.5 copies/ml.