Influence of Baf-A1 treatment on Luc knockdown efficiency by the AmB-loaded PDMA-b-PDPA micelleplexes. (A) Relative Luc knockdown efficiency of AmB-loaded micelleplexes in Baf-A1-pretreated A549-Luc cells. (B) Relative Luc knockdown efficiency vs Baf-A1 addition times. The cells were first treated with micelleplex, and then Baf-A1 was added at different time points (0, 3, 6, or 12 h) after micelleplex addition (1.0 wt % AmB loading, w/w 10, and siRNA concentration of 75 nM were applied) (*p < 0.05, **p < 0.01). Rel Luc knockdown was determined by normalizing the decreased Luc activity in siRNA-Luc-treated cells over that of the siRNA-Scr-treated control.