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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Bioorg Med Chem Lett. 2016 Feb 23;26(7):1651–1654. doi: 10.1016/j.bmcl.2016.02.069

Figure 3.

Figure 3

Calibration curve for NaN3. Fl-DIBO 100 μM, NaN3 0–100 μM in a mixture of 50 mM HEPES buffer and dioxane (1: 1) at pH 7.4. Fluorescence intensities at 469 nm were recorded after 14 h of incubation at 60°C with the addition of NaN3. Data represents the average of three independent experiments