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. Author manuscript; available in PMC: 2016 Sep 4.
Published in final edited form as: Science. 2016 Mar 4;351(6277):1090–1093. doi: 10.1126/science.aad6881

Fig. 1. Sequences upstream of TSS region affect TSS selection.

Fig. 1

(A) Promoter sequences analyzed in MASTER-N7 (2) and MASTER-N10. Promoter -35, -10, and discriminator elements are indicated. Green, randomized nucleotides.

(B) Effect of discriminator on position of TSS (numbered in bp downstream of -10 element). Data show means and 99.9% confidence intervals for each of the 64 discriminator sequences (~16,000 templates analyzed for each discriminator). Green, GGG and other RRR discriminators; blue, CCT and other YYY discriminators; red, GTG discriminator.

(C) Mean and modal TSS. *, GTG data from MASTER-N7; **, GTG data from MASTER-N10.

(D) Upstream and downstream shifts in TSS selection with the ~16,000 GGG and ~16,000 CCT discriminators (green and blue, respectively) relative to the ~16,000 GTG discriminators (red).

(E) Effect of σ1.2-discriminator interactions on TSS selection (downstream shift in mean TSS for ~16,000 GGG-discriminator templates on replacement of σ by σ1.2 mutant).