Figure 7.

CSQ2 knockdown in the RV mimics the effects of PAH. Rats were treated with in vivo siRNA for 48 h to knockdown CSQ2, and the Millar catheter was used to measure RV contractile functions. (A) CSQ2 knockdown in the RV as monitored by western blotting and (B) RV dP/dt as an indication of the contractile performance. (C) Tropomyosin, (D) troponin T, and (E) SERCA2a levels were monitored by western blotting in the RV homogenates. The symbol (*) denotes that the value is significantly different from the control value at P < 0.05 as determined by Student's t-test.