Figure 2.

The expression of proinflammatory and anti-inflammatory markers in neutrophils. (A) Representative images demonstrated that cardiac resident neutrophils (Day 0) did not proliferate, as evidenced by BrdU-negative staining. Macrophages served as positive controls. (B) The numbers of CD45⁺CD11b⁺Ly-6G⁺ cells and Ly-6G⁺ cells at Day 1 post-MI evaluated by flow cytometry. n = 6 per group. (C) Ly6G⁺ cells were positive for the neutrophil clone 7/4, but negative for the macrophage marker Mac-3. Representative images of four biological samples. (D and E) Neutrophils expressed high levels of proinflammatory markers at Day 1 and anti-inflammatory markers at Days 5 and 7 post-MI, as assessed by quantitative RT–PCR. ND, not determined (value below detection). We assigned ND as zero when performing statistical analysis. Due to the small number of neutrophils in Day 0 LVs, we pooled three to four LVs per sample. n = 4–6 samples per group. *P < 0.05 vs. Day 0 and ^#P < 0.05 vs. Day 1 post-MI. One-way ANOVA was used.