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. 2016 Feb 17;110(1):96–106. doi: 10.1093/cvr/cvw037

Figure 4.

Figure 4

SSH detection by DyLight-maleimide fluors switch method using MMTS or NEM as SH/SSH-blocking agent. (A) NEM (top panel) vs. MMTS (bottom panel) was used as SH/SSH-blocking agent in the modified DyLight-maleimide fluors switch method. NaHS-pretreated heart total homogenate samples were split into two aliquots, which are subjected to SH/SSH blocking by NEM and DyLight-600-maleimide labelling (B) or SH blocking by MMTS and DyLight-800-maleimide labelling (C), respectively.