Table 2.

SNO proteins identified by iodoTMT proteomic analysis in I/R control, PostC-NaHS, and PostC-NaHS + SNAP hearts

Protein name	ID	Peptide sequence	SNO-Cys	SNO occupancy [iodoTMTy/(iodoTMTx + iodoTMTy)]
				I/R control	PostC-NaHS	PostC-NaHS + SNAP
Aconitate hydratase	Q99KI0	VGLIGScTNSSYEDMGR	385	0.013 ± 0.001	0.032 ± 0.005*	0.048 ± 0.019*
ADP/ATP translocase 1	P48962	YFAGNLASGGAAGATSLcFVYPLDFAR	129		0.016 ± 0.004	0.031 ± 0.006**
Aspartate aminotransferase	P05202	NLDKEYLPIGGLAEFcK	106	0.028 ± 0.014	0.062 ± 0.003*	0.074 ± 0.004*
Branched-chain amino acid aminotransferase	O35855	AWIGGVGDcK	229	0.038 ± 0.017	0.082 ± 0.004*	0.120 ± 0.028*,**
Carnitine O-palmitoyltransferase 1, muscle isoform	Q924X2	GVYPGSPTSWLVVVMATVGSNYcK	75	0.015 ± 0.010	0.040 ± 0.004*	0.079 ± 0.023*,**
Creatine kinase M-type	P07310	AGHPFMWNEHLGYVLTcPSNLGTGLR	317	0.017 ± 0.003	0.063 ± 0.004*	0.110 ± 0.021*,**
Creatine kinase S-type	Q6P8J7	SEVELVQIVIDGVNYLVDcEK	397		0.025 ± 0.001	0.056 ± 0.011**
Cysteine and glycine-rich protein 3	P50462	SLESTNVTDKDGELYcK	168	0.026 ± 0.012	0.069 ± 0.006*	0.093 ± 0.004*,**
Cytochrome b-c1 complex subunit 1	Q9CZ13	YFYDQcPAVAGYGPIEQLPDYNR	453	0.017 ± 0.002	0.044 ± 0.009*	0.066 ± 0.015*,**
Cytochrome c oxidase subunit 5B	P19536	IVGcIcEEDNCTVIWFWLHKGESQR	90, 92		0.034 ± 0.002	0.062 ± 0.008**
Dihydrolipoyl dehydrogenase	O08749	AEVITcDVLLVcIGR	306, 312	0.039 ± 0.012	0.093 ± 0.015*	0.127 ± 0.017*,**
Electron transfer flavoprotein subunit-β	Q9DCW4	HSMNPFcEIAVEEAVR	42		0.032 ± 0.005	0.073 ± 0.007**
Electron transfer flavoprotein oxidoreductase	Q921G7	FcPAGVYEFVPLEQGDGFR	560		0.039 ± 0.004	0.080 ± 0.009**
ES1 protein homologue	Q9D172	VALVLSGcGVYDGTEIHEASAILVHLSR	50	0.035 ± 0.014	0.090 ± 0.010*	0.124 ± 0.008*,**
F1-ATP synthase subunit-α	Q03265	YTIVVSATASDAAPLQYLAPYSGcSMGEYFR	294	0.035 ± 0.007	0.064 ± 0.001*	0.091 ± 0.017*,**
Fructose-bisphosphate aldolase A	P05064	cPLLKPWALTFSYGR	290	0.011 ± 0.003	0.059 ± 0.004*	0.079 ± 0.010*,**
Glyceraldehyde-3-phosphate dehydrogenase	P16858	VPTPNVSVVDLTcR	245	0.020 ± 0.002	0.034 ± 0.003*	0.050 ± 0.006*,**
Heat shock protein HSP 90-β	P11499	LVSSPccIVTSTYGWTANMER	589, 590	0.010 ± 0.001	0.030 ± 0.006*	0.052 ± 0.002*,**
Heparin-binding growth factor 1	P61148	LLYcSNGGHFLR	31	0.175 ± 0.007	0.263 ± 0.061*	0.336 ± 0.025*,**
High mobility group protein B3	O54879	MSAYAFFVQTcR	23	0.040 ± 0.003	0.114 ± 0.016*	0.142 ± 0.011*,**
Histidine triad nucleotide-binding protein 1	P70349	cLAFHDISPQAPTHFLVIPK	38	0.033 ± 0.007	0.105 ± 0.016*	0.145 ± 0.012*,**
Hydroxyacyl-coenzyme A dehydrogenase	Q61425	TFESLVDFcK	201	0.179 ± 0.008	0.219 ± 0.006*	0.256 ± 0.029*,**
3-hydroxyisobutyrate dehydrogenase	Q99L13	IcNNMLLAISMIGTAEAMNLGIR	210		0.046 ± 0.008	0.082 ± 0.003**
Isocitrate dehydrogenase [NADP]	P54071	VcVQTVESGAMTK	402	0.069 ± 0.012	0.103 ± 0.005*	0.110 ± 0.011*
Long-chain specific acyl-CoA dehydrogenase	P51174	LLIAELAISAcEFMFEETR	303	0.017 ± 0.007	0.064 ± 0.011*	0.084 ± 0.005*,**
MACRO domain-containing protein 1	Q922B1	ScYLSSLDLLLEHR	244	0.032 ± 0.006	0.141 ± 0.015*	0.202 ± 0.018*,**
Malate dehydrogenase	P08249	GcDVVVIPAGVPR	93	0.018 ± 0.002	0.044 ± 0.011*	0.049 ± 0.003*
Mitochondrial carnitine/acylcarnitine carrier protein	Q9Z2Z6	LQTQPPSLSGQPPMYSGTLDcFR	58		0.029 ± 0.002	0.054 ± 0.009**
Myoglobin	P04247	HGcTVLTALGTILK	67	0.031 ± 0.004	0.089 ± 0.013*	0.118 ± 0.011*,**
Myosin-6	Q02566	MESDLTQLQTEVEEAVQEcR	1750		0.043 ± 0.004	0.076 ± 0.005**
NADH dehydrogenase [ubiquinone] flavoprotein 1	Q91YT0	LKPPFPADVGVFGcPTTVANVETVAVSPTIcR	238, 255	0.015 ± 0.004	0.033 ± 0.007*	0.068 ± 0.018*,**
Peptidyl-prolyl cis-trans isomerase F	Q99KR7	HVGPGVLSMANAGPNTNGSQFFIcTIK	156	0.014 ± 0.002	0.054 ± 0.009*	0.079 ± 0.011*,**
Peroxiredoxin-2	Q61171	KLGcEVLGVSVDSQFTHLAWINTPR	70	0.024 ± 0.011	0.073 ± 0.010*	0.083 ± 0.008*
Phosphoglucomutase-1	Q9D0F9	LSLcGEESFGTGSDHIR	374	0.042 ± 0.031	0.105 ± 0.003*	0.152 ± 0.010*,**
Profilin-1	P62962	cYEMASHLR	128	0.102 ± 0.028	0.151 ± 0.019*	0.198 ± 0.021*,**
Protein DJ-1	Q99LX0	KGLIAAIcAGPTALLAHEVGFGcK	106, 121	0.024 ± 0.010	0.089 ± 0.013*	0.113 ± 0.009*,**
Pyruvate dehydrogenase E1 component subunit β	Q9D051	TNHLVTVEGGWPQFGVGAEIcAR	306	0.014 ± 0.012	0.094 ± 0.008*	0.099 ± 0.012*
40S ribosomal protein S11	P62281	cPFTGNVSIR	60	0.073 ± 0.040	0.135 ± 0.011*	0.199 ± 0.012*,**
Short-chain specific acyl-CoA dehydrogenase	Q07417	AcASTGVIMSVNNSLYLGPILK	109	0.013 ± 0.003	0.045 ± 0.003*	0.060 ± 0.005*,**
Succinate dehydrogenase flavoprotein subunit	Q8K2B3	TYFScTSAHTSTGDGTAMVTR	266	0.019 ± 0.007	0.082 ± 0.012*	0.124 ± 0.019*,**
Trifunctional enzyme subunit α	Q8BMS1	ALMGLYNGQVLcK	349		0.033 ± 0.001	0.053 ± 0.016**
Triosephosphate isomerase	P17751	IIYGGSVTGATcK	268		0.036 ± 0.010	0.078 ± 0.014**

SNO cysteine residues (SNO-Cys) are labelled by iodoTMT_y and shown in lower case (c). Protein identifications were accepted based on two or more unique peptides with a false discovery rate (FDR) of 99% or higher. SNO-modified proteins and peptides listed in the Table were identified from each of three individual samples.

*P < 0.05 vs. I/R control.

**P < 0.05 vs. PostC-NaHS; n = 3 in each group.