LSNA, HR, and MAP were increased 1 hr following icv injection of leptin, and these changes were partially reversed by bilateral nanoinjection of SHU9119 into the PVN 30 min later. Top: Representative experiment showing icv leptin + PVN SHU9119 (at arrows). Groups are: middle, icv leptin + PVN SHU9119 (n=5); bottom, icv aCSF + PVN SHU9119 (n=6). *: P<0.05, compared to baseline control values (CON); †: P<0.05 compared to icv aCSF at the same time; ‡: P<0.05, icv leptin + PVN SHU9119 compared to icv leptin + PVN aCSF (Figure 1A).