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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 2;65:1331–1339. doi: 10.1016/j.freeradbiomed.2013.09.027

Figure 4. Induction of HO-1 increases TTP levels in macrophages.

Figure 4

(A & B) RAW 264.7 cells were treated with the indicated concentration of hemin for 18h. HO-1 levels were analyzed by Western blot (A) and semi-qRT-PCR (B) and TTP levels were analyzed by semi-qRT-PCR (B). (C) RAW 264.7 cells were pretreated with 20μM ZnPP for 30min and further incubated with 10 μM hemin for 18h. The levels of TTP and HO-1 were analyzed by semi-qRT-PCR. (D & E) RAW 264.7 cells were treated with the indicated concentration of CoPP for 18h. HO-1 levels were analyzed by Western blot (D) and semi-qRT-PCR (E) and TTP levels were analyzed by semi-qRT-PCR (E). (F) RAW 264.7 cells were pretreated with 20μM ZnPP for 30min and further incubated with 10μM CoPP for 18h. The levels of TTP and HO-1 were analyzed by semi-qRT-PCR.