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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: FEBS Lett. 2009 May 3;583(12):1933–1938. doi: 10.1016/j.febslet.2009.04.039

FIGURE 2. p38 MAPK inhibits TTP-directed decay of IL-10 mRNA.

FIGURE 2

Wild-type (A) and TTP−/− (B) BMDM were treated with LPS for 4h and then actinomycin D was added together with 1 μM SB 202190 or vehicle (0.1% DMSO). Cells were harvested at the times shown and RNA was isolated. IL-10 and GAPDH mRNAs were measured by quantitative real-time PCR using the ΔΔCt method of analysis. Graphs show IL-10 mRNA normalised to GAPDH mRNA expressed as a percentage of t=0 actinomycin D treatment from one experiment. Qualitatively similar data were obtained by RPA in two additional independent experiments.