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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: FEBS Lett. 2009 May 3;583(12):1933–1938. doi: 10.1016/j.febslet.2009.04.039

FIGURE 4. TTP binds an IL-10 mRNA 3’UTR probe in EMSA.

FIGURE 4

RAW 264.7 cells either left untreated or treated with LPS for 2h. EMSA was performed using 50 μg of cytoplasmic extract and 20 fmol of 32P labelled IL-10 3’UTR RNA probe per lane. Some samples were pre-incubated with either a rabbit antiserum against HSP27 (non-immune, NI) or an antiserum against TTP. Samples were electrophoresed on a non-denaturing acrylamide gel at 4°C and analysed by phosphorimaging. Similar results were obtained in two separate experiments.