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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: FEBS Lett. 2009 May 3;583(12):1933–1938. doi: 10.1016/j.febslet.2009.04.039

FIGURE 6. Regulation of IL-1α and IL-6 mRNA stability by p38 MAPK requires TTP.

FIGURE 6

Actinomycin D chases were performed in wild-type (A) or TTP−/− (B) BMDM as in Figure 1. Cells were harvested at the times shown and RNA was extracted and subjected to ribonuclease protection assay (RPA) with the RiboQuant multi-probe template set mCK-2b. Protected RNA fragments were detected and quantified by phosphorimaging. Graphs show mean IL-6 (C) and IL-1α mRNA (D) mRNA ± S.E.M. from four experiments in wild-type cells and mean IL-6 (E) and IL-1α mRNA (F) mRNA ± S.E.M. from three experiments in TTP−/− cells. Significance of the effect of SB 202190 on mRNA levels was measured by paired Student’s t-test *p<0.01. Where smaller than the symbols error bars are not shown.