Fig 4. Bile acid components effect biofilm formation in P. aeruginosa.

(A) CLSM images of horizontal (xy) and vertical (xz and yz) projections of biofilm structures formed by P. aeruginosa ΔwspF mutant in the presence of DMSO control or 50 μM of TLCA. Biofilms were incubated at 37°C and images were taken at 24 hours. (B) CSLM images of preformed biofilms after treatment with either DMSO control or 50 μM of TLCA for 19 hours at 37°C.