Fig 5. Comparison of multi-locus genotypes underlying differences in temperature sensitivity among BYx3S FLO8^3S ira2Δ2933 segregants.

Across our current study and past work, we have identified five predominant multi-locus genotypes that underlie the three temperature sensitivity classes. Excluding FLO8^3S, which is generally required for expression of the trait in the presence of ira2Δ2933, these genotypes involve specific alleles at seven environmentally responsive loci. Respectively, blue and orange indicate the BY or 3S allele of a given gene participates in a specific multi-locus genotype, while grey indicates that neither allele is required. Additionally, brown lines emphasize how adding particular combinations of alleles to a FLO8^3S ira2Δ2933 MSS11^BY genetic background can lead to reduction or elimination of temperature sensitivity.