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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Vis Neurosci. 2016 Jan;33:E001. doi: 10.1017/S0952523815000310

Figure 1. Computer-assisted manual marking of cell nuclei.

Figure 1

A Single image from z-stack before marking. B Single image from z-stack with Voronoi regions (surrogate for cells, thin green borders around cells) displayed and cell nuclei identified (white dots). All data within yellow edging were used for analysis. As previously described, (Ach et al., 2014) Voronoi regions (collection of regions that tessellate a plane) were generated from corresponding RPE cytoskeleton images (not shown). Scale bar: 20 μm.